Journal: Scientific Reports

Article Title: Enrichment technique to allow early detection and monitor emergence of KRAS mutation in response to treatment

doi: 10.1038/s41598-019-47700-9

Figure Lengend Snippet: Binding affinities of KRAS5 with DNAs of KRAS mutations (G12D and G12V) or KRAS wild type (WT).

Article Snippet: Biotinylated hairpin KRAS G12D mutated nucleotide (5′biotin- TACATCTGATGGCGTATTAAGTTTTCTTAATACGCCATCAGATGTA-3′, Hokkaido System Science), KRAS G12V mutated nucleotide (5′biotin- TACATCTGTTGGCGTATTAAGTTTTCTTAATACGCCAACAGATGTA-3′, Hokkaido System Science) and KRAS wild nucleotide (5′biotin- TACATCTGGTGGCGTATTAAGTTTTCTTAATACGCCACCAGATGTA-3′, Hokkaido System Science) were immobilized on a streptavidin coated SA sensor chip at a flow rate of 10 μL/min to obtain the required immobilization level (up to approximately 1000 resonance units (RU)).

Techniques: Binding Assay

Journal: Scientific Reports

Article Title: Enrichment technique to allow early detection and monitor emergence of KRAS mutation in response to treatment

doi: 10.1038/s41598-019-47700-9

Figure Lengend Snippet: Patients’ characteristics.

Article Snippet: Biotinylated hairpin KRAS G12D mutated nucleotide (5′biotin- TACATCTGATGGCGTATTAAGTTTTCTTAATACGCCATCAGATGTA-3′, Hokkaido System Science), KRAS G12V mutated nucleotide (5′biotin- TACATCTGTTGGCGTATTAAGTTTTCTTAATACGCCAACAGATGTA-3′, Hokkaido System Science) and KRAS wild nucleotide (5′biotin- TACATCTGGTGGCGTATTAAGTTTTCTTAATACGCCACCAGATGTA-3′, Hokkaido System Science) were immobilized on a streptavidin coated SA sensor chip at a flow rate of 10 μL/min to obtain the required immobilization level (up to approximately 1000 resonance units (RU)).

Techniques:

Journal: Scientific Reports

Article Title: Enrichment technique to allow early detection and monitor emergence of KRAS mutation in response to treatment

doi: 10.1038/s41598-019-47700-9

Figure Lengend Snippet: Structure of biotinylated pyrrole–imidazole polyamides. ( A ) Chemical structure of the PI polyamide, KRAS4. ( B ) Chemical structure of the PI polyamide, KRAS5. ( C ) KRAS4 targets G12S/G12C mutations. ( D ) KRAS5 targets G12V/G12D mutations.

Article Snippet: Biotinylated hairpin KRAS G12D mutated nucleotide (5′biotin- TACATCTGATGGCGTATTAAGTTTTCTTAATACGCCATCAGATGTA-3′, Hokkaido System Science), KRAS G12V mutated nucleotide (5′biotin- TACATCTGTTGGCGTATTAAGTTTTCTTAATACGCCAACAGATGTA-3′, Hokkaido System Science) and KRAS wild nucleotide (5′biotin- TACATCTGGTGGCGTATTAAGTTTTCTTAATACGCCACCAGATGTA-3′, Hokkaido System Science) were immobilized on a streptavidin coated SA sensor chip at a flow rate of 10 μL/min to obtain the required immobilization level (up to approximately 1000 resonance units (RU)).

Techniques:

Journal: Scientific Reports

Article Title: Enrichment technique to allow early detection and monitor emergence of KRAS mutation in response to treatment

doi: 10.1038/s41598-019-47700-9

Figure Lengend Snippet: Schematic representation of the enrichment assay using biotinylated pyrrole–imidazole polyamides.

Article Snippet: Biotinylated hairpin KRAS G12D mutated nucleotide (5′biotin- TACATCTGATGGCGTATTAAGTTTTCTTAATACGCCATCAGATGTA-3′, Hokkaido System Science), KRAS G12V mutated nucleotide (5′biotin- TACATCTGTTGGCGTATTAAGTTTTCTTAATACGCCAACAGATGTA-3′, Hokkaido System Science) and KRAS wild nucleotide (5′biotin- TACATCTGGTGGCGTATTAAGTTTTCTTAATACGCCACCAGATGTA-3′, Hokkaido System Science) were immobilized on a streptavidin coated SA sensor chip at a flow rate of 10 μL/min to obtain the required immobilization level (up to approximately 1000 resonance units (RU)).

Techniques: